Single-cell genome analysis
for breakthroughs in microbiome research
Whole-genome sequencing can provide valuable insights about the type and function of bacteria.
However, little whole-genome information can actually be accessed because only a small fraction of bacteria have been cultured.
We have developed a new analytical platform, bit-MAP®, which sequences individual bacterial cells to explosively uncover novel genomes through the high-throughput manner and enables us to acquire the whole genomes at the competitive price under USD30 per genome.
| Demo Data1: Gut Microbiome |
Demo Data2: Soil Microbiome |
bit-MAP® :
bitBiome Microbiome Analysis Platform
Hundreds of microbial whole genomes at a time
bit-MAP® eliminates conventional hurdles for microbiome research such as isolation, culture and complex data processing. This technology enables massively parallel whole-genome sequencing of diverse individual microbes to provide new insights into unknown strains.
Please refer to the sample dataset of gut microbiome.
High-quality genomic information from a single cell
In bit-MAP®, microbes are individually encapsulated, with multi-step biochemical reactions including cell lysis and DNA amplification occurring inside the capsules. Individual bacterial genomes are accurately sequenced by analyzing the DNA within each capsule.
Publications : Chijiiwa R et al. Microbiome. 2020. , Yoda T et al. Genome Resource Announcement. 2020. , Nishikawa Y et al. bioRxiv. 2020.
Bioinformatic analysis at single-cell resolution
Our unique bioinformatics platform accurately reconstructs microbial genomes at
the sub-strain level. It elucidates not only genetic and functional information of
individual bacteria, but also genetic differences between target and related strains.
Publications : Kogawa M et al. Scientific Reports. 2018.
Advantages of bit-MAP®
Towards a comprehensive understanding of target microbiomes
bit-MAP® achieves functional analyses of individual bacteria that would be difficult with existing technologies.
| Method | Isolation, Cultivation, and Sequencing |
16S rRNA Sequencing |
Shotgun metagenomics |
bit-MAP®: Single-cell genomics |
|---|---|---|---|---|
| Target | Isolated bacteria | Any microbial samples (incl. unculturable ones) | ||
| Pre-study | Required (over months) | Not required | ||
| Objective | Deep understanding of one type of bacteria |
Community-level analysis | Deep understanding of individual bacterial functions |
|
| Principle | Sequence the entire genome of a single microbe |
Sequence 16S rRNA | Sequence a mixture of microbial genomes genomes |
Individually sequence whole |
| Resolution | Strain | Genus | Species | Strain |
| Expected results | Whole genome sequences, I plasmid sequences, and gene lists of a single microbe |
Ratios of microbes present | Ratios of microbes and genes present |
Whole genome sequences, plasmid sequences, and gene lists of individual microbes |
| Potential bias | Small | Large | Small | Small |
| Contamination risk | Small | Large | Large | Small |
| Analytical load | Small | Small | Large | Controllable |
Applications of bit-MAP®
Widely applicable to bacterial samples
bit-MAP® can be applied to a wide range of human/animal samples (e.g., gut, oral and skin
microbiomes) and environmental samples (soil and marine).
We’re providing Single-cell genome analysis service at the competitive cost, under USD30 per genome (SAG) at 384-well plate, and the deliverable report contains 1. Raw Sequence Data 2. Quality of Draft Genome 3. Phylogenetic analysis 4. Taxonomy Identification of each draft genome. (Demo Data. Gut Microbiome)
Please contact us for any queries about particular sample types.
