Whole-genome sequencing can provide valuable insights about the type and function of bacteria.
However, little whole-genome information can actually be accessed because only a small fraction of bacteria have been cultured.
We have developed a new analytical platform, bit-MAP®, which sequences individual bacterial cells to explosively uncover novel genomes through the high-throughput manner and enables us to acquire the whole genomes at the competitive price under USD30 per genome.
bit-MAP® eliminates conventional hurdles for microbiome research such as isolation, culture and complex data processing. This technology enables massively parallel whole-genome sequencing of diverse individual microbes to provide new insights into unknown strains.
Please refer to the sample dataset of gut microbiome.
In bit-MAP®, microbes are individually encapsulated, with multi-step biochemical reactions including cell lysis and DNA amplification occurring inside the capsules. Individual bacterial genomes are accurately sequenced by analyzing the DNA within each capsule.
Publications : Chijiiwa R et al. Microbiome. 2020. , Yoda T et al. Genome Resource Announcement. 2020. , Nishikawa Y et al. bioRxiv. 2020.
Our unique bioinformatics platform accurately reconstructs microbial genomes at
the sub-strain level. It elucidates not only genetic and functional information of
individual bacteria, but also genetic differences between target and related strains.
Publications : Kogawa M et al. Scientific Reports. 2018.
bit-MAP® achieves functional analyses of individual bacteria that would be difficult with existing technologies.
|Target||Isolated bacteria||Any microbial samples (incl. unculturable ones)|
|Pre-study||Required (over months)||Not required|
|Objective||Deep understanding of
one type of bacteria
|Community-level analysis||Deep understanding of
individual bacterial functions
|Principle||Sequence the entire genome
of a single microbe
|Sequence 16S rRNA||Sequence a mixture
of microbial genomes genomes
|Individually sequence whole|
|Expected results||Whole genome sequences,
I plasmid sequences, and gene
lists of a single microbe
|Ratios of microbes present||Ratios of microbes
and genes present
|Whole genome sequences,
and gene lists of
bit-MAP® can be applied to a wide range of human/animal samples (e.g., gut, oral and skin
microbiomes) and environmental samples (soil and marine).
We’re providing Single-cell genome analysis service at the competitive cost, under USD30 per genome (SAG) at 384-well plate, and the deliverable report contains 1. Raw Sequence Data 2. Quality of Draft Genome 3. Phylogenetic analysis 4. Taxonomy Identification of each draft genome. (Demo Data. Gut Microbiome)
Please contact us for any queries about particular sample types.