bitBiome has developed a “viable bacteria-targeted single-cell genome sequencing (PMA-SAG-gel method)” that sequences and analyzes only viable bacteria from human commensal bacteria such as gut microbiome and skin microbiome, and we have published the details of the research used to analyze human intestinal bacteria in Scientific Reports.
Title: Strain-level profiling of viable microbial community by selective single-cell genome sequencing
The newly developed PMA-SAG-gel method can examine the survival profile of bacterial flora at the strain level.
By using the PMA-SAG-gel method in this study, we found that different strains of the same bacterial species have different survival rates under certain conditions. This suggests that strain-level evaluation is necessary to obtain accurate information on viable bacteria.
We expect that this method will provide useful information on the characterization of viable bacteria in the bacterial flora and on the evaluation of the quality of enteric bacteria in viable bacterial preparations and fecal transplants, leading to a better understanding of the impact of bacteria on human health.
The results of this research were published in the open access scientific journal Scientific Reports on March 15, 2022.
＜Paper information＞
Title of Paper：Strain-level profiling of viable microbial community by selective single-cell genome sequencing
Journal：Scientific Reports
Main Author： Taruho Endo, Masahito Hosokawa
Latest Article Publication Date: March 15, 2022
DOI: https://doi.org/10.1038/s41598-022-08401-y